Modular synthesis of de novo-designed metalloproteins for light-induced electron transfer
نویسندگان
چکیده
منابع مشابه
Modular synthesis of de novo-designed metalloproteins for light-induced electron transfer.
The design and chemical synthesis of two de novo four-helix bundle proteins is described; each protein has two bound cofactors. Their construction from purified peptides is based on the modular assembly of different amphiphilic helices by chemoselective coupling to a cyclic peptide template. In the hydrophobic interior of the antiparallel four-helix bundle these proteins contain a heme in a bin...
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A de novo designed coiled-coil metalloprotein was prepared that uses electrostatic interactions to control both its conformational and bimolecular electron-transfer properties. The title protein exists as a coiled-coil heterodimer of the [Ru(trpy)(bpy)-KK(37-mer)] and [Ru(NH(3))(5)-EE(37-mer)] polypeptides which is formed by interhelix electrostatic attractions. Circular dichroism studies show ...
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The close functional interplay between the prosthetic group and the polypeptide chain in redox enzymes is the theme of this paper, and re— presents a natural extension of the well established regulation exerted by the protein mojety on the ligand binding properties of hemoglobins and other oxygen carriers. Two relevant examples are discussed: in the first one (azurin) the mole— cular mechanism ...
متن کاملDe novo designed peptides for biological applicationsw
In recent years our ability to design and assemble peptide-based materials and objects de novo (i.e. from first principles) has improved considerably. This brings us to a point where the resulting assemblies are quite sophisticated and amenable to engineering in new functions. Whilst such systems could be used in a variety of ways, biological applications are of particular interest because of t...
متن کاملDe novo design and structural characterization of proteins and metalloproteins.
De novo protein design has recently emerged as an attractive approach for studying the structure and function of proteins. This approach critically tests our understanding of the principles of protein folding; only in de novo design must one truly confront the issue of how to specify a protein's fold and function. If we truly understand proteins, it should be possible to design receptors, enzym...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1998
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.95.20.11526